human oct4-GFP

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相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: human oct4-GFP 
出品公司: 爱迪基因
目录编号: 21153
存储实验室: Rudolf Jaenisch

载体骨架基本信息
载体名称: BluescriptKSII
载体出品公司: stratagene
载体抗性: 氨苄霉素
空载体大小: 2381 bp
完整质粒大小: 122387 bp
载体类型: 哺乳细胞表达
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: hOCT4-GFP
物种来源: 人源
插入大小: 120006 bp
基因又名: hCol1a1 targeting arms
克隆方法: 限制性内切酶
5' 克隆位点: XhoI(未破坏)
3' 克隆位点: XbaI (未破坏)
5' 测序引物 CGATAAGCTTGGTACCGAGC
3' 测序引物 CCAACACACTATTGCAATGAAA

文献引用方法
材料和方法部分: human oct4-GFP was a gift from Rudolf Jaenisch (爱迪基因 plasmid # 21153)
参考文献部分: Nanoparticles for gene transfer to human embryonic stem cell colonies. Green JJ, Zhou BY, Mitalipova MM, Beard C, Langer R, Jaenisch R, Anderson DG. Nano Lett. 2008 Oct . 8(10):3126-30. 10.1021/nl8012665 PubMed 18754690

载体图谱质粒图谱和多克隆位点信息

human oct4-GFP质粒图谱

载体简介

载体序列

LOCUS       human oct4-GFP               14407 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    human oct4-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14407)
  AUTHORS   Green JJ, Zhou BY, Mitalipova MM, Beard C, Langer R, Jaenisch R, 
            Anderson DG
  TITLE     Nanoparticles for gene transfer to human embryonic stem cell 
            colonies.
  JOURNAL   Nano Lett. 2008 Oct . 8(10):3126-30.
  PUBMED    18754690
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|human oct4-GFP|
FEATURES             Location/Qualifiers
     source          1..14407
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        2544..3040
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2579..2602)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2973..2992
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3080..3679
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3080..3099)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3576..3596
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4215..4231
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4216..4232
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8307..9023
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8352..8373)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8613..8632)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8960..8981
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          9055..9627
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(12040..12059)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12041..12059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12146..12163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12317..12905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12397..12416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13076..13936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13699..13718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13937..14041)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14109..14127
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14165..14187)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14287..14306
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        14387..14405
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14387..14404
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tcgaggtgga caccaccctc aagagcctga gccagcagat cgagaacatc cggagcccag
       61 agggcagccg caagaacccc gcccgcacct gccgtgacct caagatgtgc cactctgact
      121 ggaagagtgg tgagggcctg ccctagcctc tccctccctc ctactcctgc catgccaggg
      181 tccccatgcc catatgtgcc cctaccatat ggtgctggct gctccctttc cctgactcca
      241 tcttgccctg ccctaccaca ggagagtact ggattgaccc caaccaaggc tgcaacctgg
      301 atgccatcaa agtcttctgc aacatggaga ctggtgagac ctgcgtgtac cccactcagc
      361 ccagtgtggc ccagaagaac tggtacatca gcaagaaccc caaggacaag aggcatgtct
      421 ggttcggcga gagcatgacc gatggattcc aggtgcgtga gctggacctc agagccagtg
      481 ttaggagatg ggctagccca gtgctcagaa gggacatgaa gtcctggagt aggtctctgc
      541 taagggtgat ggacagagct gggctgggag gcaggggtct caggtccctg atagtggttc
      601 agacacaggc tgccgatggg caggtggtgc ctcctctcga tataacggtg cattgggcag
      661 ctctctgagg accctggaca gggaggccag gcaggactag aggttcccgc atagcggctc
      721 actcttccct ctctcccctc ccctgcagtt cgagtatggc ggccagggct ccgaccctgc
      781 cgatgtggcc atccagctga ccttcctgcg cctgatgtcc accgaggcct cccagaacat
      841 cacctaccac tgcaagaaca gcgtggccta catggaccag cagactggca acctcaagaa
      901 ggccctgctc ctccagggct ccaacgagat cgagatccgc gccgagggca acagccgctt
      961 cacctacagc gtcactgtcg atggctgcac ggtgagtgcc cagaatcccc aggcagggcc
     1021 ccacctctcc ggccttgggc tttttggcca ggccatagtg ccctctctcc atcactccca
     1081 cgtggtaatg ccccctcccg ttgtctccgc cccaccccag agtcacaccg gagcctgggg
     1141 caagacagtg attgaataca aaaccaccaa gacctcccgc ctgcccatca tcgatgtggc
     1201 ccccttggac gttggtgccc cagaccagga attcggcttc gacgttggcc ctgtctgctt
     1261 cctgtaaact ccctccatcc caacctggct ccctcccacc caaccaactt tccccccaac
     1321 ccggaaacag acaagcaacc caaactgaac cccctcaaaa gccaaaaaat gggagacaat
     1381 ttcacatgga ctttggaaaa tatttttttc ctttgcattc atctctcaaa cttagttttt
     1441 atctttgacc aaccgaacat gaccaaaaac caaaagtgca ttcaacctta ccaaaaaaaa
     1501 aaaaaaaaaa agaataaata aataactttt taaaaaagga agcttggtcc acttgcttga
     1561 agacccatgc gggggtaagt ccctttctgc ccgttgggct tatgaaaccc caatgctgcc
     1621 ctttctgctc ctttctccac accccccttg gggcctcccc tccactcctt cccaaatctg
     1681 tctccccaga agacacagga aacaatgtat tgtctgccca gcaatcaaag gcaatgctca
     1741 aacacccaag tggcccccac cctcagcccg ctcctgcccg cccagcaccc ccaggccctg
     1801 ggggacctgg ggttctcaga ctgccaaaga agccttgcca tctggcgctc ccatggctct
     1861 tgcaacatct ccccttcgtt tttgaggggg tcatgccggg ggagccacca gcccctcact
     1921 gggttcggag gagagtcagg aagggccacg acaaagcaga aacatcggat ttggggaacg
     1981 cgtgtcaatc ccttgtgccg cagggctggg cgggagagac tgttctgttc cttgtgtaac
     2041 tgtgttgctg aaagactacc tcgttcttgt cttgatgtgt caccggggca actgcctggg
     2101 ggcggggatg ggggcagggt ggaagcggct ccccatttta taccaaaggt gctacatcta
     2161 tgtgatgggt ggggtgggga gggaatcact ggtgctatag aaattgagat gcccccccag
     2221 gccagcaaat gttccttttt gttcaaagtc tatttttatt ccttgatatt tttctttttt
     2281 tttttttttt tttgtggatg gggacttgtg aatttttcta aaggtgctat ttaacatggg
     2341 aggagagcgt gtgcggctcc agcccagccc gctgctcact ttccaccctc tctccacctg
     2401 cctctggctt ctcaggcctc tgctctccga cctctctcct ctgaaaccct cctccacagc
     2461 tgcagcccat cctcccggct ccctcctagt ctgtcctgcg tcctctgtcc ccgggtaccg
     2521 ggccccccct cgaccattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     2581 atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     2641 cattccacat ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     2701 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     2761 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     2821 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     2881 gggctcagca gctgggaagg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     2941 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     3001 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct
     3061 cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     3541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     3601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     3661 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     3721 acgaccccat gcatcgatga tatcagatcc ccgggatgca gaaattgatg atctattaaa
     3781 caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag
     3841 aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg
     3901 attagataaa tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc
     3961 atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc
     4021 actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca
     4081 ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag
     4141 atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt
     4201 ccatcagaag ctggtcgagg tcgacggtat cgataagctt ggtaccgagc tcggatccac
     4261 tagtaacggc cgccagtgtg ctggaattcg ccctcagggt acagggaggc ctatgccaac
     4321 ttctcattga acaccactcc cagctttcct acccccattt ctcttcacag gtgtgcagag
     4381 aagtctacat tcccatgtca agtaaggggg gtgggtagat gggagggcat atgagagcgg
     4441 ggcagcaatt ttatgtctga gttggaagga agtttagagt tcattgagtc caaatcctct
     4501 ttactaggtg aggaaactga ggcacaggag ggggaggtgc cattagccag ggagtcagag
     4561 ccaggactta gaacctggtc catgcctcct agtttgttcc tgaccaatcc tcccagctca
     4621 cccactcctt ttgctctcac ctctgactcc ctacaggcta cagctagaga gttgactcct
     4681 ctatttgagg taagagtgtc tggatttggg ataaggtgac ctcagtttgc agggctcatg
     4741 ggggagatca gagggtcctt tgttgagaag aggggacttg gtccacagtg agcaaaggaa
     4801 tttgtctaaa ttgctattaa gggagatcat ctcccaaggt gggccaagcc tgaaccctca
     4861 gccctccgtt tcttcccttt cttttgcctt cagattgaca ggcctcggaa gtcaaaataa
     4921 gtggtttcct agaccgggtc gagagcaagt ctctattggt cccaactgag ttttttcagc
     4981 tggtttttca accaaacagc acctcatctc ccagtgaggg gaagggaagg ctgggctgag
     5041 agcagcaagg ctgctcatct cacctctccc cacccagcca tgccagccgc ctcacctggt
     5101 ggggagaggt gggcctcacc tgggtcccct ggcagtgctc tgtgaagggt cttgacattg
     5161 cactgtaata ataaaggtgt gtgtgaagta tctttttatg gtgactttct aaaacccagg
     5221 gaatcatggg accagttctg atgactcagc ctggcttcca gtctcctcca ggcccaacgg
     5281 tggcccccag cactggttgg ggcctgggag agctggcctt ggctgaagtg aagccaccta
     5341 cccttcaggc ataacaggac agtgagaagg aaggaaagcc tgcctcaacc tcccatcagc
     5401 cctgagcacc ccagaagggg gccggctagg agtctaggca tgcaggaggc tgacccctga
     5461 ctgggctcat atccagccac aaggcagcca gggatccagg cacccacccc ttgtctgcgt
     5521 ccctctcggg aatgggcctc ttgcccaggc cagaaataca ccacctacag tacaaattat
     5581 aatctaaaaa caagagggtg gtgttgagtg gggaaattgg ggaaggtgtt ttaggagcca
     5641 ctaggaaaat gggcagcagg gactctctgg actggcttgg gaagagcgct tttggggaac
     5701 ctggaggatg gcaagctgag aaacactggt gtggagattc cagccaaatc ccaggcctgc
     5761 ccctccccct cctctgagag gccgtcttct tggcagacag cagagagatg catgacaaag
     5821 gtgccgtgat ggttctgtcc tggggattga gatggctggg gaggggcctc ctcctgttcc
     5881 gaagcatgtt cctcccaccc ccaccaggcc ccataatcta cctgcctttt gggcagttaa
     5941 aggccgagaa gtgaacacag ctgcaacccc actgccttgt agaccttccg gcagacctgt
     6001 ggcaggtatt gaaatgcacg catacaatta ggctcaaaaa gtctacacag acaggagatg
     6061 ggcacacgaa cagaggcaac ataagagtgg gggaaaagtc tcaaaagact cacggatgcc
     6121 accaagatga agacagctgg ccacgggaca cccatcccct tagaaggcag atagagccac
     6181 tgaccccagc agacaagccc aggcagggct gagcctggag cctgcaatga gaagccttac
     6241 ttaagtcgac agaggtcagc gtgcccagtc cagacctggc cttctggcct tcgaagctgt
     6301 ggggagccct ggcccagagc cccctctgga gcccccagac ttaccccagg ccctccactg
     6361 agatcaagtt ttgggagcag acagacaaac atcatccctc acagacaggc attccgttgg
     6421 ctattctctt gcaaacagaa tcaagcacta gaccagcagc atgagcctca ggatactcag
     6481 gccaggccca gaaaaacaga ccctgaaggg gagcttaggg cagccttcct gcacgcctcc
     6541 acaaatcact ctccacctcc tctgcgtctt tctgccagcc agccccacta aacaaagcac
     6601 atccctcaat ctgccaggct cggggaggga cgcacgatga agctggacgc ctgagtcccc
     6661 cagaggaagg aggaactaga tacctaggtc cctgtggggg gcccttggtg cccgtctgag
     6721 gctcagtctt tgaggggatt gcagaggggg gttgctggag ctccttttag cgtctctgaa
     6781 ggggattctg tgtgagggga ttgggactgg ggggttgggg agcaggaagc agtccccagg
     6841 ggagccatcc aggcccattc aagggttgag cacttgttta gggttagagc tgccccctct
     6901 ggggaccggg attgtccagc caaggccatt gtcctgcccc cttcccccag tccctcccag
     6961 gcttctttga acctgaagtc agatattttt tctccacacc ccccaccccc tggttttccc
     7021 cacccagggc ctagggctgg aggcctgggc cagggaggtg ggggagggag aacggggcct
     7081 accgtggtat tagatgtctg agttttggtt gagaggggag caaggaacct gatgtgcagg
     7141 ttccatagtg gagggggccc aaagcgggtg tcttatcact ctgtttcagc aaaggttggg
     7201 aaactgaggc ccagtcagtc caaagtctgg tcccttgaag gggaagtagg gaccaacccc
     7261 ttagtctgtt agatgaggag agtctggagt ctgattctgg aagacggagg ggtgggggga
     7321 tgggggggtg gggggatata gcacggaggc cttgtctggc agtctactct tgaagatggg
     7381 gtgaaatttg gcaggctggg cagatggtgc caggcaccca ggctgcgggg tggctggatt
     7441 tggccagtat cgggatggga atgcctagga ttctggatgg atcgggggaa ggcataaggg
     7501 agcagctggc cattgtgctt atggctgttg atgcattgag ggatagcgcc acacacacat
     7561 tcaataaatt tgaggagctg agagggtgac tggcccctga aggcacagtg ccagaggtct
     7621 gtggagaggg ggtcaagcac ctgggttcct gaagaacatg gaggtgtggg agtgattcca
     7681 gacagctggg atgtgcagag cctgagagag tgccagggag cgggttggga gttgaaagtt
     7741 gggtgtggtg gctcacgcct ttaatcatga cactgggcgg cagaggcggg aggatttctt
     7801 gaggacagga attcaagacc agcctgggta acatagcaag gccccatctc tactaaaaat
     7861 aaaaaaacta acagggcaca gtggtccaag cctgtagtcc cagccactta ggaggctgga
     7921 gcagaaggat tgctttggcc cagtagatcg aggctacatt gagccatcat tgtactccac
     7981 tgcactccag tctgggcaac aaagtgagac cctgtcttaa aaaataaaaa taaaaaaagt
     8041 ttctgtgggg gacctgcact gaggtcctgg aggggcgcca gttgtgtctc ccggttttcc
     8101 ccttccacag acaccattgc caccaccatt aggcaaacat ccttcgcctc agtttctccc
     8161 cccacctccc tctcctccac ccatccaggg ggcggggcca gaggtcaagg ctagtgggtg
     8221 ggactgggga gggagagagg ggttgagtag tcccttcgca agccctcatt tcaccaggcc
     8281 cccggcttgg ggcgccttcc ttccccatgg tgagcaaggg cgaggagctg ttcaccgggg
     8341 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     8401 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     8461 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     8521 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     8581 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     8641 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     8701 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     8761 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     8821 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     8881 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     8941 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     9001 tcggcatgga cgagctgtac aagactagtt attaaggatc ctgagaactt cagggtgagt
     9061 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
     9121 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
     9181 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
     9241 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
     9301 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
     9361 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     9421 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     9481 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     9541 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     9601 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
     9661 catcattttg gcaaaggatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     9721 ggtgtggcca atgccctggc tcacaaatac cactgagatc gttttccctc tgccaaaaat
     9781 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     9841 ttcattgcaa tagtgtgttg gaatttctcg atcgctagag cggccgccac cgcggtggag
     9901 ctcagagaca acttcccaaa gcacaaagca gtttttcccc ctaggggtgg gaggaagcaa
     9961 aagactctgt acctattttg tatgtgtata ataatttgag atgtttttaa ttattttgat
    10021 tgctggaata aagcatgtgg aaatgaccca aacataatcc gcagtggcct cctaatttcc
    10081 ttctttggag ttgggggagg ggtagacatg gggaaggggc tttggggtga tgggcttgcc
    10141 ttccattcct gccctttccc tccccactat tctcttctag atccctccat aaccccactc
    10201 ccctttctct cacccttctt ataccgcaaa cctttctact tcctctttca ttttctattc
    10261 ttgcaatttc cttgcacctt ttccaaatcc tcttctcccc tgcaatacca tacaggcaat
    10321 ccacgtgcac aacacacaca cacactcttc acatctgggg ttgtccaaac ctcataccca
    10381 ctccccttca agcccatcca ctctccaccc cctggatgcc ctgcacttgg tggcggtggg
    10441 atgctcatgg atactgggag ggtgagggga gtggaacccg tgaggaggac ctgggggcct
    10501 ctccttgaac tgacatgaag ggtcatctgg cctctgctcc cttctcaccc acgctgacct
    10561 cctgccgaag gagcaacgca acaggagagg ggtctgctga gcctggcgag ggtctgggag
    10621 ggaccaggag gaaggcgtgc tccctgctcg ctgtcctggc cctgggggag tgagggagac
    10681 agacacctgg gagagctgtg gggaaggcac tcgcaccgtg ctcttgggaa ggaaggagac
    10741 ctggccctgc tcaccacgga ctgggtgcct cgacctcctg aatccccaga acacaacccc
    10801 cctgggctgg ggtggtctgg ggaaccatcg tgcccccgcc tcccgcctac tcctttttaa
    10861 gctttttctt tctttctttc ttttttttct taaattcttg cttcctcttc ccccctcttc
    10921 tccgtccctt ctctttccct tcaccttctg cctgctcctt cccagaggcc ctgagaaggc
    10981 gaggcccgga gggaaggtga ctgttcggct cccccatctc aggtaagggg gacagcgagt
    11041 gtggtcagcg ggggacagag aatccagggc gtcagaagtc cagagagaac caggcacccg
    11101 gagccaggga gacagcgaga cgacagctgg tcacctctct cacctcatct caggtctaaa
    11161 cccaaaaaga acaaaaagcc cgcactcctc cctaaggtct ccagagagat ggacaagtct
    11221 gtagatacca tccatcagca atactaatat cgtgatgtca ttaagtattc atggccgttc
    11281 attatggcct tcaataattc atcattagca cttctaccac acttaggaca gtgtagaaag
    11341 ggaatcagtg tagagaggat aagaatgatg gcatataaat gaaggcttat tcatatgcaa
    11401 attacccaaa gttgtgtgat gcctatccat ctctctttct ctcttttttt tgagacagag
    11461 tctcactctg tcacccaggc tggagtgcgg tggcgcagtc tcagctcact gcaacctccg
    11521 cctcccaggt tcaagcaatt ctcatgcctc agcctcccaa gtaactggga tgacagccat
    11581 gcactaccac gcccagctaa tttttatatt tttagtagag tcagggtttt accatgttag
    11641 ccagcctggt ctcgaactcc tggcctcaag tgatccacct gcctcagcct ctcaaagtgc
    11701 tgggattgcg gacatgagcc acagcaccag gctccatctc ttgatcaatg tgttgattgc
    11761 ttggtgatgc ctgccatgct gcccaggaaa ccctgtccac ttggacctct gggactttag
    11821 gaccctggct gtccaggcca catccccatc tgccctcgtg ggggacccag ggacatcctg
    11881 agcctggcat ccaaggctct acctggccac tacccacctg cccaggctca ctgtcccctc
    11941 ccctgcccac atctgtcaca ccaaccaaaa agggtggcca acagttctca caaatgcact
    12001 gcatttcccc acttccaggc cttctagatc tgccggtctc cctatagtga gtcgtattaa
    12061 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    12121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    12181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    12241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    12301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    12361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    12421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    12481 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
    12541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    12601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    12661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    12721 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    12781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    12841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    12901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    12961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    13021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    13081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    13141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    13201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    13261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    13321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    13381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    13441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    13501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    13561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    13621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    13681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    13741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    13801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    13861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    13921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    13981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    14041 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    14101 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    14161 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    14221 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    14281 actatgcggc atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag
    14341 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
    14401 atagaac
//

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