质粒名称: | human oct4-GFP |
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出品公司: | 爱迪基因 |
目录编号: | 21153 |
存储实验室: | Rudolf Jaenisch |
载体骨架基本信息 |
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载体名称: | BluescriptKSII |
载体出品公司: | stratagene |
载体抗性: | 氨苄霉素 |
空载体大小: | 2381 bp |
完整质粒大小: | 122387 bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | hOCT4-GFP |
物种来源: | 人源 |
插入大小: | 120006 bp |
基因又名: | hCol1a1 targeting arms |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | XhoI(未破坏) |
3' 克隆位点: | XbaI (未破坏) |
5' 测序引物 | CGATAAGCTTGGTACCGAGC |
3' 测序引物 | CCAACACACTATTGCAATGAAA |
文献引用方法 |
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材料和方法部分: | human oct4-GFP was a gift from Rudolf Jaenisch (爱迪基因 plasmid # 21153) |
参考文献部分: | Nanoparticles for gene transfer to human embryonic stem cell colonies. Green JJ, Zhou BY, Mitalipova MM, Beard C, Langer R, Jaenisch R, Anderson DG. Nano Lett. 2008 Oct . 8(10):3126-30. 10.1021/nl8012665 PubMed 18754690 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS human oct4-GFP 14407 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS human oct4-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14407) AUTHORS Green JJ, Zhou BY, Mitalipova MM, Beard C, Langer R, Jaenisch R, Anderson DG TITLE Nanoparticles for gene transfer to human embryonic stem cell colonies. JOURNAL Nano Lett. 2008 Oct . 8(10):3126-30. PUBMED 18754690 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|human oct4-GFP| FEATURES Location/Qualifiers source 1..14407 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 2544..3040 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(2579..2602) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 2973..2992 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 3080..3679 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3080..3099) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3576..3596 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind 4215..4231 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 4216..4232 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS 8307..9023 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(8352..8373) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8613..8632) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8960..8981 /label=EGFP-C /note="EGFP, forward primer" intron 9055..9627 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind complement(12040..12059) /label=T7 /note="T7 promoter, forward primer" promoter complement(12041..12059) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(12146..12163) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(12317..12905) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(12397..12416) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(13076..13936) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 13699..13718 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(13937..14041) /gene="bla" /label=AmpR promoter primer_bind 14109..14127 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(14165..14187) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 14287..14306 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 14387..14405 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 14387..14404 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 tcgaggtgga caccaccctc aagagcctga gccagcagat cgagaacatc cggagcccag 61 agggcagccg caagaacccc gcccgcacct gccgtgacct caagatgtgc cactctgact 121 ggaagagtgg tgagggcctg ccctagcctc tccctccctc ctactcctgc catgccaggg 181 tccccatgcc catatgtgcc cctaccatat ggtgctggct gctccctttc cctgactcca 241 tcttgccctg ccctaccaca ggagagtact ggattgaccc caaccaaggc tgcaacctgg 301 atgccatcaa agtcttctgc aacatggaga ctggtgagac ctgcgtgtac cccactcagc 361 ccagtgtggc ccagaagaac tggtacatca gcaagaaccc caaggacaag aggcatgtct 421 ggttcggcga gagcatgacc gatggattcc aggtgcgtga gctggacctc agagccagtg 481 ttaggagatg ggctagccca gtgctcagaa gggacatgaa gtcctggagt aggtctctgc 541 taagggtgat ggacagagct gggctgggag gcaggggtct caggtccctg atagtggttc 601 agacacaggc tgccgatggg caggtggtgc ctcctctcga tataacggtg cattgggcag 661 ctctctgagg accctggaca gggaggccag gcaggactag aggttcccgc atagcggctc 721 actcttccct ctctcccctc ccctgcagtt cgagtatggc ggccagggct ccgaccctgc 781 cgatgtggcc atccagctga ccttcctgcg cctgatgtcc accgaggcct cccagaacat 841 cacctaccac tgcaagaaca gcgtggccta catggaccag cagactggca acctcaagaa 901 ggccctgctc ctccagggct ccaacgagat cgagatccgc gccgagggca acagccgctt 961 cacctacagc gtcactgtcg atggctgcac ggtgagtgcc cagaatcccc aggcagggcc 1021 ccacctctcc ggccttgggc tttttggcca ggccatagtg ccctctctcc atcactccca 1081 cgtggtaatg ccccctcccg ttgtctccgc cccaccccag agtcacaccg gagcctgggg 1141 caagacagtg attgaataca aaaccaccaa gacctcccgc ctgcccatca tcgatgtggc 1201 ccccttggac gttggtgccc cagaccagga attcggcttc gacgttggcc ctgtctgctt 1261 cctgtaaact ccctccatcc caacctggct ccctcccacc caaccaactt tccccccaac 1321 ccggaaacag acaagcaacc caaactgaac cccctcaaaa gccaaaaaat gggagacaat 1381 ttcacatgga ctttggaaaa tatttttttc ctttgcattc atctctcaaa cttagttttt 1441 atctttgacc aaccgaacat gaccaaaaac caaaagtgca ttcaacctta ccaaaaaaaa 1501 aaaaaaaaaa agaataaata aataactttt taaaaaagga agcttggtcc acttgcttga 1561 agacccatgc gggggtaagt ccctttctgc ccgttgggct tatgaaaccc caatgctgcc 1621 ctttctgctc ctttctccac accccccttg gggcctcccc tccactcctt cccaaatctg 1681 tctccccaga agacacagga aacaatgtat tgtctgccca gcaatcaaag gcaatgctca 1741 aacacccaag tggcccccac cctcagcccg ctcctgcccg cccagcaccc ccaggccctg 1801 ggggacctgg ggttctcaga ctgccaaaga agccttgcca tctggcgctc ccatggctct 1861 tgcaacatct ccccttcgtt tttgaggggg tcatgccggg ggagccacca gcccctcact 1921 gggttcggag gagagtcagg aagggccacg acaaagcaga aacatcggat ttggggaacg 1981 cgtgtcaatc ccttgtgccg cagggctggg cgggagagac tgttctgttc cttgtgtaac 2041 tgtgttgctg aaagactacc tcgttcttgt cttgatgtgt caccggggca actgcctggg 2101 ggcggggatg ggggcagggt ggaagcggct ccccatttta taccaaaggt gctacatcta 2161 tgtgatgggt ggggtgggga gggaatcact ggtgctatag aaattgagat gcccccccag 2221 gccagcaaat gttccttttt gttcaaagtc tatttttatt ccttgatatt tttctttttt 2281 tttttttttt tttgtggatg gggacttgtg aatttttcta aaggtgctat ttaacatggg 2341 aggagagcgt gtgcggctcc agcccagccc gctgctcact ttccaccctc tctccacctg 2401 cctctggctt ctcaggcctc tgctctccga cctctctcct ctgaaaccct cctccacagc 2461 tgcagcccat cctcccggct ccctcctagt ctgtcctgcg tcctctgtcc ccgggtaccg 2521 ggccccccct cgaccattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 2581 atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca 2641 cattccacat ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac 2701 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga 2761 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc 2821 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct 2881 gggctcagca gctgggaagg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 2941 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 3001 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct 3061 cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 3121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 3181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 3241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 3301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 3361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 3421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 3481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 3541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 3601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 3661 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc 3721 acgaccccat gcatcgatga tatcagatcc ccgggatgca gaaattgatg atctattaaa 3781 caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag 3841 aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg 3901 attagataaa tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc 3961 atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc 4021 actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca 4081 ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag 4141 atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt 4201 ccatcagaag ctggtcgagg tcgacggtat cgataagctt ggtaccgagc tcggatccac 4261 tagtaacggc cgccagtgtg ctggaattcg ccctcagggt acagggaggc ctatgccaac 4321 ttctcattga acaccactcc cagctttcct acccccattt ctcttcacag gtgtgcagag 4381 aagtctacat tcccatgtca agtaaggggg gtgggtagat gggagggcat atgagagcgg 4441 ggcagcaatt ttatgtctga gttggaagga agtttagagt tcattgagtc caaatcctct 4501 ttactaggtg aggaaactga ggcacaggag ggggaggtgc cattagccag ggagtcagag 4561 ccaggactta gaacctggtc catgcctcct agtttgttcc tgaccaatcc tcccagctca 4621 cccactcctt ttgctctcac ctctgactcc ctacaggcta cagctagaga gttgactcct 4681 ctatttgagg taagagtgtc tggatttggg ataaggtgac ctcagtttgc agggctcatg 4741 ggggagatca gagggtcctt tgttgagaag aggggacttg gtccacagtg agcaaaggaa 4801 tttgtctaaa ttgctattaa gggagatcat ctcccaaggt gggccaagcc tgaaccctca 4861 gccctccgtt tcttcccttt cttttgcctt cagattgaca ggcctcggaa gtcaaaataa 4921 gtggtttcct agaccgggtc gagagcaagt ctctattggt cccaactgag ttttttcagc 4981 tggtttttca accaaacagc acctcatctc ccagtgaggg gaagggaagg ctgggctgag 5041 agcagcaagg ctgctcatct cacctctccc cacccagcca tgccagccgc ctcacctggt 5101 ggggagaggt gggcctcacc tgggtcccct ggcagtgctc tgtgaagggt cttgacattg 5161 cactgtaata ataaaggtgt gtgtgaagta tctttttatg gtgactttct aaaacccagg 5221 gaatcatggg accagttctg atgactcagc ctggcttcca gtctcctcca ggcccaacgg 5281 tggcccccag cactggttgg ggcctgggag agctggcctt ggctgaagtg aagccaccta 5341 cccttcaggc ataacaggac agtgagaagg aaggaaagcc tgcctcaacc tcccatcagc 5401 cctgagcacc ccagaagggg gccggctagg agtctaggca tgcaggaggc tgacccctga 5461 ctgggctcat atccagccac aaggcagcca gggatccagg cacccacccc ttgtctgcgt 5521 ccctctcggg aatgggcctc ttgcccaggc cagaaataca ccacctacag tacaaattat 5581 aatctaaaaa caagagggtg gtgttgagtg gggaaattgg ggaaggtgtt ttaggagcca 5641 ctaggaaaat gggcagcagg gactctctgg actggcttgg gaagagcgct tttggggaac 5701 ctggaggatg gcaagctgag aaacactggt gtggagattc cagccaaatc ccaggcctgc 5761 ccctccccct cctctgagag gccgtcttct tggcagacag cagagagatg catgacaaag 5821 gtgccgtgat ggttctgtcc tggggattga gatggctggg gaggggcctc ctcctgttcc 5881 gaagcatgtt cctcccaccc ccaccaggcc ccataatcta cctgcctttt gggcagttaa 5941 aggccgagaa gtgaacacag ctgcaacccc actgccttgt agaccttccg gcagacctgt 6001 ggcaggtatt gaaatgcacg catacaatta ggctcaaaaa gtctacacag acaggagatg 6061 ggcacacgaa cagaggcaac ataagagtgg gggaaaagtc tcaaaagact cacggatgcc 6121 accaagatga agacagctgg ccacgggaca cccatcccct tagaaggcag atagagccac 6181 tgaccccagc agacaagccc aggcagggct gagcctggag cctgcaatga gaagccttac 6241 ttaagtcgac agaggtcagc gtgcccagtc cagacctggc cttctggcct tcgaagctgt 6301 ggggagccct ggcccagagc cccctctgga gcccccagac ttaccccagg ccctccactg 6361 agatcaagtt ttgggagcag acagacaaac atcatccctc acagacaggc attccgttgg 6421 ctattctctt gcaaacagaa tcaagcacta gaccagcagc atgagcctca ggatactcag 6481 gccaggccca gaaaaacaga ccctgaaggg gagcttaggg cagccttcct gcacgcctcc 6541 acaaatcact ctccacctcc tctgcgtctt tctgccagcc agccccacta aacaaagcac 6601 atccctcaat ctgccaggct cggggaggga cgcacgatga agctggacgc ctgagtcccc 6661 cagaggaagg aggaactaga tacctaggtc cctgtggggg gcccttggtg cccgtctgag 6721 gctcagtctt tgaggggatt gcagaggggg gttgctggag ctccttttag cgtctctgaa 6781 ggggattctg tgtgagggga ttgggactgg ggggttgggg agcaggaagc agtccccagg 6841 ggagccatcc aggcccattc aagggttgag cacttgttta gggttagagc tgccccctct 6901 ggggaccggg attgtccagc caaggccatt gtcctgcccc cttcccccag tccctcccag 6961 gcttctttga acctgaagtc agatattttt tctccacacc ccccaccccc tggttttccc 7021 cacccagggc ctagggctgg aggcctgggc cagggaggtg ggggagggag aacggggcct 7081 accgtggtat tagatgtctg agttttggtt gagaggggag caaggaacct gatgtgcagg 7141 ttccatagtg gagggggccc aaagcgggtg tcttatcact ctgtttcagc aaaggttggg 7201 aaactgaggc ccagtcagtc caaagtctgg tcccttgaag gggaagtagg gaccaacccc 7261 ttagtctgtt agatgaggag agtctggagt ctgattctgg aagacggagg ggtgggggga 7321 tgggggggtg gggggatata gcacggaggc cttgtctggc agtctactct tgaagatggg 7381 gtgaaatttg gcaggctggg cagatggtgc caggcaccca ggctgcgggg tggctggatt 7441 tggccagtat cgggatggga atgcctagga ttctggatgg atcgggggaa ggcataaggg 7501 agcagctggc cattgtgctt atggctgttg atgcattgag ggatagcgcc acacacacat 7561 tcaataaatt tgaggagctg agagggtgac tggcccctga aggcacagtg ccagaggtct 7621 gtggagaggg ggtcaagcac ctgggttcct gaagaacatg gaggtgtggg agtgattcca 7681 gacagctggg atgtgcagag cctgagagag tgccagggag cgggttggga gttgaaagtt 7741 gggtgtggtg gctcacgcct ttaatcatga cactgggcgg cagaggcggg aggatttctt 7801 gaggacagga attcaagacc agcctgggta acatagcaag gccccatctc tactaaaaat 7861 aaaaaaacta acagggcaca gtggtccaag cctgtagtcc cagccactta ggaggctgga 7921 gcagaaggat tgctttggcc cagtagatcg aggctacatt gagccatcat tgtactccac 7981 tgcactccag tctgggcaac aaagtgagac cctgtcttaa aaaataaaaa taaaaaaagt 8041 ttctgtgggg gacctgcact gaggtcctgg aggggcgcca gttgtgtctc ccggttttcc 8101 ccttccacag acaccattgc caccaccatt aggcaaacat ccttcgcctc agtttctccc 8161 cccacctccc tctcctccac ccatccaggg ggcggggcca gaggtcaagg ctagtgggtg 8221 ggactgggga gggagagagg ggttgagtag tcccttcgca agccctcatt tcaccaggcc 8281 cccggcttgg ggcgccttcc ttccccatgg tgagcaaggg cgaggagctg ttcaccgggg 8341 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 8401 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 8461 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 8521 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 8581 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 8641 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 8701 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 8761 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 8821 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 8881 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 8941 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 9001 tcggcatgga cgagctgtac aagactagtt attaaggatc ctgagaactt cagggtgagt 9061 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg 9121 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc 9181 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt 9241 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt 9301 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa 9361 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt 9421 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt 9481 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat 9541 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat 9601 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct 9661 catcattttg gcaaaggatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct 9721 ggtgtggcca atgccctggc tcacaaatac cactgagatc gttttccctc tgccaaaaat 9781 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 9841 ttcattgcaa tagtgtgttg gaatttctcg atcgctagag cggccgccac cgcggtggag 9901 ctcagagaca acttcccaaa gcacaaagca gtttttcccc ctaggggtgg gaggaagcaa 9961 aagactctgt acctattttg tatgtgtata ataatttgag atgtttttaa ttattttgat 10021 tgctggaata aagcatgtgg aaatgaccca aacataatcc gcagtggcct cctaatttcc 10081 ttctttggag ttgggggagg ggtagacatg gggaaggggc tttggggtga tgggcttgcc 10141 ttccattcct gccctttccc tccccactat tctcttctag atccctccat aaccccactc 10201 ccctttctct cacccttctt ataccgcaaa cctttctact tcctctttca ttttctattc 10261 ttgcaatttc cttgcacctt ttccaaatcc tcttctcccc tgcaatacca tacaggcaat 10321 ccacgtgcac aacacacaca cacactcttc acatctgggg ttgtccaaac ctcataccca 10381 ctccccttca agcccatcca ctctccaccc cctggatgcc ctgcacttgg tggcggtggg 10441 atgctcatgg atactgggag ggtgagggga gtggaacccg tgaggaggac ctgggggcct 10501 ctccttgaac tgacatgaag ggtcatctgg cctctgctcc cttctcaccc acgctgacct 10561 cctgccgaag gagcaacgca acaggagagg ggtctgctga gcctggcgag ggtctgggag 10621 ggaccaggag gaaggcgtgc tccctgctcg ctgtcctggc cctgggggag tgagggagac 10681 agacacctgg gagagctgtg gggaaggcac tcgcaccgtg ctcttgggaa ggaaggagac 10741 ctggccctgc tcaccacgga ctgggtgcct cgacctcctg aatccccaga acacaacccc 10801 cctgggctgg ggtggtctgg ggaaccatcg tgcccccgcc tcccgcctac tcctttttaa 10861 gctttttctt tctttctttc ttttttttct taaattcttg cttcctcttc ccccctcttc 10921 tccgtccctt ctctttccct tcaccttctg cctgctcctt cccagaggcc ctgagaaggc 10981 gaggcccgga gggaaggtga ctgttcggct cccccatctc aggtaagggg gacagcgagt 11041 gtggtcagcg ggggacagag aatccagggc gtcagaagtc cagagagaac caggcacccg 11101 gagccaggga gacagcgaga cgacagctgg tcacctctct cacctcatct caggtctaaa 11161 cccaaaaaga acaaaaagcc cgcactcctc cctaaggtct ccagagagat ggacaagtct 11221 gtagatacca tccatcagca atactaatat cgtgatgtca ttaagtattc atggccgttc 11281 attatggcct tcaataattc atcattagca cttctaccac acttaggaca gtgtagaaag 11341 ggaatcagtg tagagaggat aagaatgatg gcatataaat gaaggcttat tcatatgcaa 11401 attacccaaa gttgtgtgat gcctatccat ctctctttct ctcttttttt tgagacagag 11461 tctcactctg tcacccaggc tggagtgcgg tggcgcagtc tcagctcact gcaacctccg 11521 cctcccaggt tcaagcaatt ctcatgcctc agcctcccaa gtaactggga tgacagccat 11581 gcactaccac gcccagctaa tttttatatt tttagtagag tcagggtttt accatgttag 11641 ccagcctggt ctcgaactcc tggcctcaag tgatccacct gcctcagcct ctcaaagtgc 11701 tgggattgcg gacatgagcc acagcaccag gctccatctc ttgatcaatg tgttgattgc 11761 ttggtgatgc ctgccatgct gcccaggaaa ccctgtccac ttggacctct gggactttag 11821 gaccctggct gtccaggcca catccccatc tgccctcgtg ggggacccag ggacatcctg 11881 agcctggcat ccaaggctct acctggccac tacccacctg cccaggctca ctgtcccctc 11941 ccctgcccac atctgtcaca ccaaccaaaa agggtggcca acagttctca caaatgcact 12001 gcatttcccc acttccaggc cttctagatc tgccggtctc cctatagtga gtcgtattaa 12061 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 12121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 12181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 12241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 12301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 12361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 12421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 12481 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 12541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 12601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 12661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 12721 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 12781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 12841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 12901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 12961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 13021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 13081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 13141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 13201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 13261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 13321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 13381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 13441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 13501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 13561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 13621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 13681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 13741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 13801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 13861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 13921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 13981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 14041 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 14101 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 14161 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 14221 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 14281 actatgcggc atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag 14341 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact 14401 atagaac //
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